Black cumin nigella sativa and its constituent thymoquinone. Effect of ethanolic extracts of spices named nigella sativa, allium sativum and syzygium aromaticum against pseudomonas aeruginosa in vitro 11 2 2 3arifa jabeen, quratulain hanif, shehar bano, maryam riaz and ali ahmed sheikh 1department of microbiology, university of veterinary and animal sciences, lahore, pakistan. Antimicrobial effect of nigella sativa seed extract. All neonates were divided and examined into two experimental and control groups randomly.
Antibacterial and antifungal activity assesment of nigella. Antiinflammatory, analgesic and antipyretic activity of nigella sativa. The in vivo antimicrobial effect of the nigella sativa seeds extract at a concentration of 33% on pustules staphylococcal skin infections was assessed and compared with standard drug mupirocin on 40 neonates. In the present study antibacterial effect of aqueous extract of seeds was studied against few of clinical isolates including both gram po results. Only nigella sativa extract alone showed synergistic effect with antibiotics and the positive results were showed by s. All media plates 9 cm in diameter were prepared with mullerhinton agar. Essential oil eo of nigella sativa has a broad antimicrobial spectrum. The antibacterial activity of the volatile oil of nigella sativa seeds was studied against 37 isolates of shigella dysenteriae 1, shigella flexneri, shigella sonnei and shigella boydii and 10 strains of vibrio cholerae and escherichia coli.
Antimicrobial activity of black cumin seeds nigella sativa. Antibacterial and antioxidant activity of nigella sativa. Formulation, characterization and antimicrobial properties of. Pdf in vitro antimicrobial activity of nigella sativa. The antimicrobial effects of nigella sativa have extensively been studied in vitro 15,16, 29, 31,43,60,74 and in vivo 66 against various. The effect of nigella sativa extract, on streptococcus faecalis, escherichia. Seed extracts from six species of the genus nigella family ranunculaceaenigella arvensis, nigella damascena, nigella hispanica, nigella nigellastrum, nigella orientalis, and nigella sativaobtained by successive extraction with nhexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution. Evaluation of the antimicrobial activity of aqueous. The antibacterial activity of thymoquinone tq and its biofilm inhibition potencies were investigated on 11 human pathogenic bacteria. Bacterial biofilms tend to exhibit significant tolerance to antimicrobials drugs during infections. The equipment used were cleaned and sterilized by immersing them in ethanol for 10 min then washed with distill waterd. Gas chromatography mass spectrometry was run to specify their chemical composition. Sativa seeds were evaluated by agarwell diffusion method. Pdf antimicrobial activity of black cumin extracts.
Apr, 2011 thymoquinone is an active principle of nigella sativa seed known as habbah alsauda in arabic countries and sinouj in tunisia. The present study was aimed at determining the chemical constituents of nigella sativa seeds and their anticancer and antibacterial activities. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds pcymene, thymoquinone, carvacrol, and thymol of n. Chemical composition, antimicrobial potential against. Consequently, black cumin oil may be used as an antimicrobial agent in food products to prevent spoilage. Analysis and antibacterial activity of nigella sativa.
Gcms analysis of nigella sativa seeds and antimicrobial braz. Supercritical fluid extraction, nigella sativa, antimicrobial activity, gram positive and gram negative bacteria, university putra malaysia, yemen introduction r the extraction of essential oil components using. Studies on the antimicrobial activity of nigella sativa. Pharmaceutical research center, faculty of pharmacy, 65. Nigella sativa is a herbaceous plant found in the middle east, europe and western and middle asia. Production of new potent agents is urgently needed, especially for hospitals and health care centers. Pdf antimicrobial activity of black cumin seeds nigella. In this study, antibacterial activity of nigella sativa seed extract against some pathogenic bacterial strains streptococcus pyogene, pseudomonas aeruginosa, klebseilla pneumoniae and proteus vulgaris was evaluated. Pdf wide spectrum antibacterial activity of nigella sativa l.
Journal of medicinal plants research antibacterial. The antimicrobial properties of herbal plants and their extracts have been recognized since ancient times, while attempts to illustrate these. Mics of garanda, black seed and tualang honey ranged from 5. Evaluation of the synergistic antimicrobial effect of.
Dermatological effects of nigella sativa sciencedirect. Dec 30, 2010 nigella sativa ns seeds have been used for medicinal purposes for centuries both as herbs and its oil. Known by the common name black cumin is a medicinal plant from the ranunculaceae family. Antimicrobial activity of nigella sativa seed extract. Effect of ethanolic extracts of spices named nigella sativa. Gcms analysis of nigella sativa seeds and antimicrobial. An in vitro study model, authormd saifur rahman and md.
Extraction of essential oil from nigella sativa using. Poonam prakash department of chemistry shiats, allahabad, india abstract. Antibacterial activity of thymoquinone, an active principle. The strength of inhibition and the spectrum of antimicrobial activity of n. Phytochemical analysis of nigella sativa and its antibacterial and anticancer activities muqdad khairi yaheya dr. Hatem departments of pharmacology and microbiology, faculty of veterinary medicine, cairo university, cairo. The delicate flowers have 510 petals and the colors are usually yellow, white, pink, pale blue or pale purple. This study aimed to analyze the antimicrobial activity of black cumin nigella sativa extracts in inhibiting the growth of pathogenic and spoilage bacteria. Antimicrobial activity of nigella sativa seed extract ukm. Comparison of chemical composition and antibacterial. The aqueous and organic extracts of different germinating stages of nigella sativa seeds were. Graph 1,2,3,4 and fig 1,2 nigella sativa essential oils methanol extracts have more antibacterial and antifungal properties than etheric ones.
This study is designed to explore antimicrobial effect of extract from the nigella sativa seeds against skin pustules infection. D ctrd is a leading cause of sexually transmitted diseases. New antimicrobial agents are urgently needed to overcome this problem. A modified disc diffusion method was used for determination of the antibacterial activities of the nigella sativa ground seeds with water nsgsw, and the following steps were carried out. Methanol extract of nigella sativa exhibited significant antibacterial activity at the concentration of 50 mgml p. Effect of nigella sativa the black seed on immunity. Nigella sativa seeds contain 3638% fixed oils, proteins, alkaloids, saponin and 0. It is a small shrub with tapering green leaves and rosaceous white and purplish flowers. The antimicrobial activity from ethanol and petroleum ether extracts against multiple microorganisms was re ported 8. New antimicrobial agents are urgently needed to overcome. Anticancer activity of nigella sativa black seed a. Antimicrobial activity of nigella sativa against bacteria incubator for a day at 37oc. Journal of ethnopharmacology, 34 1991 27578 elsevier scientific publishers ireland ltd.
Pathogenic bacterial infections have become a major health problem worldwide. Nigella sativa black cumin seed oil and methanolic extract were tested in varying dilutions against strains of staphylococcus epidermidis and other coagulasenegative staphylococci resistant to a number of clinically used antibiotics isolated from. For this purpose, extracts of nigella sativa seeds were prepared in ethyl acetate, methanol, acetone, hexane, diethyl ether, chloroform. Antibacterial activity of the essential oils of nigella sativa l. In vitro antimicrobial activity of ethanolic seeds extracts of nigella sativa linn in the sudan.
Evaluation of phytochemical and antibacterial activity of nigella sativa l. Nigella sativa ns seeds have been used for medicinal purposes for centuries both as herbs and its oil. Aug 31, 2017 harzallah hj, bochra k, guido f, amina b, touhami m 2011 chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of tunisian nigella sativa essential oil and thymoquinone. Analysis and antibacterial activity of nigella sativa essential oil formulated in microemulsion system j. Detection of the antibacterial effect of nigella sativa. Studies on the antimicrobial activity of nigella sativa seed black. Our findings were in agreement with literature reporting thymoquinone, the most active constituent, to have substantial antimicrobial activity against mrsa.
Its ripe fruit contains tiny seeds, dark black in color, known as habba alsauda or habba albarakah in arabic and black seed in. Fazly bazzaz2, and maryam motevaly haghi3 1correspondence author. Composition, in vitro antioxidant and antimicrobial. Hanene jrah harzallah, bochra kouidhi, guido flamini, amina bakhrouf and touhami mahjoub, chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of tunisian nigella sativa essential oil and thymoquinone, food chemistry, 129, 4, 1469, 2011. Antiyeast activity of each extract was checked by agar well diffusion method against saccharomyces. Antibacterial activity of total extracts and essential oil. In vitro antibacterial activity of the volatile oil of. Filter paper discs impregnanted with the diethyl ether extract of nigella sativa seeds 25400. Antimicrobial activity of black cumin seed nigella sativa l. Ranunculaceae, commonly known as black seed or black cumin has been used for medicinal purposes for.
The aim of the present research was to find out the antibacterial activity of nigella sativa and piper nigrum against the 2 gram positive and 3 gram negative bacteria. Detection of the antibacterial effect of nigella sativa ground seeds. Nigella sativa oil as well as methanolic extract have been found to possess remarkable antibacterial activity against multidrug resistant coagulase negative staphylococci. Pdf antibacterial activity of nigella sativa seed extracts. Antimicrobial activity of black cumin seeds nigella. Huge number of studies have been carried out in recent years on the pharmacological effects of these seeds and also the possible relationship with their constituents.
Its seeds have a great medicinal importance and have been reported to exhibit many pharmacological effects that include antiparasitic, antibacterial, antifungal, antiviral, antioxidant and antiinflammatory activities. A well was made in another plate which was filled with 40 ul of the solvent only. A well was made in another plate which was filled with 40 ul of ampicillin as a positive control and incubated in similar ways. Pdf evaluation of antibacterial activity of piper betel. Seed extract of nigella sativa contains various phytochemicals having antibacterial, antifungal and medicinal properties. Moreover, significant antimicrobial activity against multidrugresistant clinical bacterial isolates has been also reported. Antimitotic and antibacterial effects of the nigella. The antimicrobial and resistance modifying activities of. We investigate in this work the chemical composition by gceims, the antibacterial and the cytotoxic activities of tunisian nigella sativa essential oil and its bioactive compound, thymoquinone, were tested against various clinical cariogenic bacteria n 30. Development of novel topical cosmeceutical formulations from. The present research was carried out in the microbiology research laboratory. Pdf in vitro antimicrobial activity of ethanolic seeds. Conclusion it may be concluded from this study that n. Widely used in traditional medicine and as a food condiment in the arab world.
Pdf antibacterial activity of nigella sativa and piper. An alarming increase in bacterial strains resistant to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to current antimicrobials. Thus, this study was undertaken to develop novel gel formulations from seeds of nigella sativa l. Development of novel topical cosmeceutical formulations. Pdf antimicrobial activity of nigella sativa seed extract aktiviti. In the present study, an excellent activity of the n. Antibacterial activities of the honeys were standardized according to phenol equivalence. In this study several seed extracts from nigella sativa l. Their antiyeast properties have not yet been investigated thoroughly.
Evaluation of antimicrobial and antiinflammatory activities. Alteration in antibacterial potential of nigella sativa l. Antibacterial activity of honey and nigella sativa l. Chemical constituents and antimicrobial and antioxidant. Extraction of essential oil from nigella sativa using supercritical carbon dioxide. Pdf abstract pathogenic bacterial infections have become a major health problem worldwide. The antibacterial activity of seed extracts was initially screened against s. Antibacterial activity of the essential oils of nigella. Studies on the antimicrobial activity of nigella sativa seed. Samanthi evaluation of phytochemical and antibacterial.
Nigella sativa extract showed antibacterial synergism with streptomycin and gentamicin. The extract was prepared by reflux extraction method. For this purpose, extracts of nigella sativa seeds were. Hence, the present study was deliberately aimed with a nanoparticles using seeds of nigella sativa as a reducing. Topozada 1965 reported antibacterial activity of the phenolic fraction of n. The agar well diffusion method was used to test the antibacterial activity of nigella sativa l. Nigella sativa essential oils with high concentration of 30 ug carvacrol and thymol are more sensible against bacterias then those with lower concentrations 10 ug maybe because they are responsible of the antibacterial activity. The results provide justification for the use of the plants in folk medicine to treat various infectious diseases. Thymoquinone is an active principle of nigella sativa seed known as habbah alsauda in arabic countries and sinouj in tunisia. The aim of the study was to evaluate the chemical composition and antibacterial activity of the oil of nigella sativa black seed against escherichia coli o157. Antibacterial activity of thymoquinone and thymohydroquinone. Mohd tariq salman1, ra khan1, i shukla2 departments of pharmacology1 and microbiology2, jawaharlal nehru medical college, aligarh muslim university, aligarh 202002.
This study was designed to examine the in vitro antimicrobial activity of nigella sativa seed by the paper disc agar diffusion method. Exploring antiyeast activity of nigella sativa seed. The nigella sativa extract prevented bacterial growth. Nigella sativa, antibacterial activity, gcms introduction infectious diseases pose serious problems to health and they are main cause of morbidity and mortality worldwide1. Exhibiting good antimicrobial activity, nigella sativa l. Sh msu d, 2h f z 3r as e dab u l h 1l ab or ty im u nh ep i cd v, sf b 2d ep ar tm nof m i cb lg y dp s, f u h h s 3d ep artm nof m icb lg y dp h,f u v.
Nigella sativa oil was then studied for antibacterial activity against these multidrug resistant isolates of staphylococcus aureus in varying. Petri dishes with 20 ml of mueller hinton agar were prepared and streaked with bacterial strains. Sultan mohideen abstract plants have been documented for their medicinal potential which is in use by the traditional healers and in indian system of medicine namely, ayurveda, unani and siddha. Disc diffusion and in agar dilution methods were performed to assess the antibacterial activity. Volume 34, issues 23, september 1991, pages 275278. Essential oils extracted from five fiber varie ties of. Wide spectrum antibacterial activity of nigella sativa l. This study was designed to evaluate the antibacterial activity of algerian honey and some extracts of nigella sativa l. Gramnegative bacteria represented by pseudomonas aeruginosa and escherichia coli but not salmonella typhimurium and a pathogenic yeast candida albicans. Thymoquinone, pcymene monoterpene, longifolene sesquiterpene, and thymohydroquinone were responsible for strong antimicrobial activity of black cumin oil.
Antibacterial effect of turkish black cumin nigella sativa l. Chloroform and ethanol extracts of black cumin seeds nigella sativa were analyzed for antibacterial activity against five food and water borne pathogens, namely, staphylococcus aureus, bacillus cereus, vibrio cholerae, klebsiella pneumoniae, escherichia coli, and one food spoilage bacteria bacillus subtilis, all of which were previously found to be resistant to different antibiotics. Abbouni laboratory for molecular microbiology proteomics and health, department of biology, faculty of natural science and life, university of djillali liabes of sidi bel abbes, 22000. Antimicrobial activity of black cumin seeds nigella sativa against multidrug resistant strains of coagulase negative staphylococci. Nowadays, multiple drug resistance has developed due to indiscriminate use of commercial antimicrobial drugs commonly used in treatment of. Bioactive compounds of nigella sativa essential oil as. Mohd tariq salman1, ra khan1, i shukla2 departments of pharmacology1 and microbiology2, jawaharlal nehru medical. Comparison of chemical composition and antibacterial activity of nigella sativa seed essential oils obtained by different extraction methods l. Oleoresins have high concentration of unsaturated fatty acids along.
Some investigations performed to show the possibility of antimicrobial and antibacterial activities of these seeds using their extracts or oil ali. But till date, up to our knowledge, there is no report on synthesis of silver nanoparticles from nigella sativa. Seeds of nigella sativa have been used for a long time in folk medicine for the treatment of skin infections. Antimicrobial activity of nigella sativa seed extract aktiviti antimikrob ekstrak nigella sativa article pdf available in sains malaysiana 422. In islam it is regarded as one of the greatest forms of healing medicine included in the medicine of prophet mohammed. Focus on antimicrobial effects, different extracts of n.
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